郑州大学学报（医学版）Issue(5)：602-605,4.DOI:10.3969/j.issn.1671-6825.2013.05.008
β-连环素对人羊膜间充质干细胞增殖的影响
Influence of β-catenin on the proliferation of human AMSC
摘要
Abstract
Aim:To observe the influence of β-catenin on the proliferation of human amniotic membrane derived mes-enchymal stem cells ( AMSC) .Methods:The adenovirus carrying human β-catenin-GFP was amplified and then transfected into human AMSC , followed by observation of the cells by fluorescent microscope .The life span was detected by CKK-8 methods.Results:The expression of β-catenin-GFP fusion protein was confirmed by immunofluorescence studies .Com-pared with the control normal cell , the proliferation rate of AMSCs whith high β-catenin expression was obviously higher , respectively, 50.0%, 82.5%, 92.6%, 169.1%and 128.2%.Conclusion: The fusion protein can be successfully ex-pressed in human AMSC .The high expression of β-catenin can effectively stimulate AMSC proliferation .关键词
β-连环素/重组腺病毒/羊膜间充质干细胞Key words
β-catenin/recombinant adenovirus/AMSC分类
医药卫生引用本文复制引用
张一折,田毅,杨波,马轩,关方霞,芦俊萍,张东丽,梁硕,迟连凯,赵永辉,王满仓,何涛,乔露华..β-连环素对人羊膜间充质干细胞增殖的影响[J].郑州大学学报（医学版）,2013,(5):602-605,4.基金项目
国家自然科学基金资助项目81071008；河南省杰出青年基金资助项目114100510005；河南省教育厅科学技术研究重点项目13A180656 （）
