郑州大学学报（医学版）Issue(6)：777-781,5.DOI:10.13705/j.issn.1671-6825.2015.06.012
Notch1和 HES1在骨肉瘤组织中的表达及其对 U2 OS 细胞侵袭能力的影响
Expressions of Notch1 and HES1 in osteosarcoma tissue and their effects on invasion ability of osteosarcoma cell U2OS
摘要
Abstract
Aim:To investigate the expressions of Notch 1 and HES1 gene in osteosarcoma tissue and their effects on invasion ability of osteosarcoma cell U 2OS.Methods:Twenty-three matched tissue specimens of osteosarcoma and matched para-tumor normal tissue, and osteosarcoma cell line U2OS and human osteoblast cell line hFOB1.19 were chosen.The ex-pressions of Notch1 and HES1 mRNA and protein in tissue specimens were detected by immunohistochemistry ,Real-time PCR and Western blot.The expressions of Notch1 and HES1 mRNA and protein in hFOB1.19,U2OS, and U2OS transfect-ed with Notch1-siRNA were detected by Real-time PCR and Western blot , and the cell invasion ability was detected by Tr-answell chambers .Results:Compared with para-tumor normal tissue , the Notch1 and HES1 mRNA and protein expressions in osteosarcoma tissue were higher(tmatch =22.567,14.830 and 8.439,7.100,P<0.001), and those in U2OS were higher than in hFOB1.19(t=11.053,20.482 and 28.845,34.681,P<0.001).Compared with U2OS, the Notch1 and HES1 mRNA and protein expressions in U2OS transfected with Notch1-siRNA were lower(t=6.294,9.209 and 9.151,13.953, P<0.05), and the invasion ability decreased(t=71.387,P<0.001).Conclusion: Notch1 and HES1 gene show high expression in both tissue and cellular level in osteosarcoma;Notch1 silence can reduce the invasion ability of U 2OS.关键词
Notch1/HES1/骨肉瘤/侵袭Key words
Notch1/HES1/osteosarcoma/invasion分类
医药卫生引用本文复制引用
王勇,赵伟,马骥..Notch1和 HES1在骨肉瘤组织中的表达及其对 U2 OS 细胞侵袭能力的影响[J].郑州大学学报（医学版）,2015,(6):777-781,5.基金项目
国家自然科学基金项目81502333；辽宁省教育厅科学研究一般项目2014418；沈阳市卫生局青年基金项目辽科发［2013］31号；沈阳医学院青年科学基金项目20132041；沈阳医学院科学研究基金项目20151002 （）
