郑州大学学报（医学版）Issue(6)：785-789,5.DOI:10.13705/j.issn.1671-6825.2015.06.014
子宫内膜腺癌组织中 IGFBP-rP1基因的甲基化状态及其 mR-NA、蛋白表达水平的检测
Detection of methylation of IGFBP-rP1 gene and relationship with its mR-NA and protein expressions in endometrial adenocarcinoma tissue
摘要
Abstract
Aim:To investigate the methylation of insulin like growth factor binding protein-related protein 1( IGFBP-rP1 ) gene and its relationship with the expressions of IGFBP-rP1 mRNA and protein in endometrial adenocarcinoma tissue . Methods:Methylation-specific PCR was used to detect the methylation status of IGFBP-rP1 gene in the promoter region and the first exon region in 45 cases of endometrial adenocarcinoma ,30 cases of endometrial atypical hyperplasia , and 30 cases of simple endometrial hyperplasia tissue , and quantitative Real-time PCR and immunohistochemical SP method were respectively used to detect the mRNA and protein expressions of IGFBP-rP1.Results: The methylation rate of IGFBP-rP1 gene in the promoter region was higher than that in the first exon region in the endometrial adenocarcinoma tissue (χ2 =6.429,P=0.011).The methylation rate of IGFBP-rP1 gene in the promoter region of endometrial adenocarcinoma tissue was significantly lower than those in endometrial atypical hyperplasia and simple endometrial hyperplasia tissue ( F =14.659,P=0.001).The mRNA and protein expressions of IGFBP-rP1 gene in endometrial adenocarcinoma tissue were higher than those in endometrial atypical hyperplasia and simple endometrial hyperplasia tissue (F=8.619,χ2 =23.611, P<0.05).The expression of IGFBP-rP1 mRNA in the endometrial adenocarcinoma tissue with the promoter methylation was lower than that without (t=4.758,P=0.001);the expression of IGFBP-rP1 protein in the endometrial adenocarcinoma tissue with the promoter methylation was negatively associated with the promoter methylation (rP =-0.625,P<0.001). Conclusion:The methylation of IGFBP-rP1 gene mainly exists in the promoter region of endometrial adenocarcinoma tissue in a low methylation status;the low methylation status in the promoter region of IGFBP-rP1 may be one of the regulatory mechanisms of the high expression of IGFBP-rP1 gene in endometrial adenocarcinoma .关键词
子宫内膜腺癌/胰岛素样生长因子结合蛋白相关蛋白1/DNA甲基化Key words
uterine endometrial adenocarcinoma/insulin like growth factor binding protein-related protein 1/DNA methylation分类
医药卫生引用本文复制引用
郭瑞霞,范丽君,李艳敏..子宫内膜腺癌组织中 IGFBP-rP1基因的甲基化状态及其 mR-NA、蛋白表达水平的检测[J].郑州大学学报（医学版）,2015,(6):785-789,5.基金项目
河南省卫生科技创新型人才工程基金资助项目豫卫科［2010］52号 （）
