郑州大学学报（医学版）Issue(6)：790-793,4.DOI:10.13705/j.issn.1671-6825.2015.06.015
PPARγ上调对 PCOS 患者卵巢颗粒细胞 P450 arom mRNA 的表达及 T 转化效应的影响
Effects of up-regulation of PPARγon expression of P450 arom mRNA and T transforming effects in ovarian granulosa cells from patients with PCOS
摘要
Abstract
Aim:To explore the effects of up-regulation of PPARγon expression of P450arom mRNA and T transfor-ming effects in the ovarian granulosa cells from patients with polycystic ovarian syndrome ( PCOS) .Methods:A total of 30 patients were included, with 10 in NA-PCOS group(PCOS with normal androgen),10 in HA-PCOS group(PCOS with hy-perandrogenism) and 10 in control group.Granulosa cells were prepared and treated with 0,1,10 nmol/L rosiglitazone for 48 h.The expressions of PPARγmRNA and P450arom mRNA were measured by RT-PCR.After intervention, the E2 value in broth was determined by ELISA and T transforming rate was calculated .Results:After the up-regulation of PPARγ, the expression of P450arom mRNA and the T transforming rate in the 3 groups were decreased,especially in the HA-PCOS group.In HA-PCOS group(being treated by 10 nmol/L rosiglitazone),the expression of PPARγmRNA was negatively as-sociated with T transforming rate,and the expression of P450arom mRNA was positively associated with T transforming rate (r=-0.633,0.829,P<0.05).Conclusion: PPARγmay participate in the pathogenesis of PCOS with high androgen level by down-regulating the expression of P450arom.关键词
多囊卵巢综合征/卵巢颗粒细胞/过氧化物酶体增殖物激活受体γ/细胞色素P450芳香化酶/T转化效应Key words
polycystic ovarian syndrome/ovarian granulosa cell/peroxisome proliferator activated receptor γ/cyto-chrome P450 aromatase/T transforming effect分类
医药卫生引用本文复制引用
王碧君,郭艺红,张慧红,李婧,杨新红..PPARγ上调对 PCOS 患者卵巢颗粒细胞 P450 arom mRNA 的表达及 T 转化效应的影响[J].郑州大学学报（医学版）,2015,(6):790-793,4.基金项目
河南省科技攻关省部共建项目201201001 （）
