化工进展Issue(5):1122-1126,5.DOI:10.3969/j.issn.1000-6613.2013.05.027
黏红酵母CCZU-G5选择性加氢柠檬醛合成香叶醇
Selective hydrogenation of citral to geraniol using Rhodotorula mucilaginosa CCZU-G5
摘要
Abstract
Biological conversion of citral to geraniol was studied using Rhodotorula mucilaginosa CCZU-G5 as fermentation strain. The effects of culture time,co-substrate concentration,initial substrate concentration,cell quantity and the reaction time on selective hydrogenation of citral to geraniol were investigated to determine the optimal fermentation conditions. The results showed that after 28 h culturing,yeast cells reached the end of the logarithmic growth phase,in which the quantity and activity of the cells were at a high level. In the process of biological conversion,the optimal fermentation conditions were as follows:glucose concentration 40 g/L,initial citral concentration 4 g/L,yeast cell concentration 0.4 g/mL and reaction time 22 h. Under these conditions,citral conversion and geraniol selectivity reached 88.6% and 95.2%,respectively. The study lays the foundation for commercialized biosynthesis of geraniol.关键词
黏红酵母/柠檬醛选择性加氢/香叶醇Key words
Rhodotorula mucilaginosa/selective hydrogenation of citral/geraniol分类
生物科学引用本文复制引用
朱劼,汪云,李晨旸,符梦婷,王利群,何玉财..黏红酵母CCZU-G5选择性加氢柠檬醛合成香叶醇[J].化工进展,2013,(5):1122-1126,5.基金项目
江苏省高校自然科学研究项目(12KJB530001)及常州市生物医药科技专项(CE20115053)。 ()