化工进展Issue(9)：2194-2199,6.DOI:10.3969/j.issn.1000-6613.2013.09.031
一株聚-β-羟基丁酸酯高产菌株的筛选及发酵条件的优化
Screening and fermentation of a high yield strain for poly-β-hydroxybutyrate
摘要
Abstract
To obtain a strain which can use inexpensive carbon source to synthesize poly-β-hydroxybutyrate(PHB) in a short time , a bacterium CCZU-6X with high production of poly-β-hydroxybutyrate (PHB) was isolated and purified from soil , which was identified as Rhodococcus sp. according to its morphological,physiological properties and 16S rDNA sequence analysis. The optimum fermentation conditions were found as follows:the most suitable carbon source was 10 g/L lactose,and nitrogen source was 2 g/L ammonium sulfate,initial pH 7.5,250 mL flask containing 30 mL medium,30 ℃,shaking at 160 r/min for 18 h. Under the optimum conditions,the production of PHB was 4.54 g/L,as high as 79.52%of dried cell weight.关键词
聚-β-羟基丁酸酯/红球菌属/发酵/优化Key words
poly-β-hydroxybutyrate/Rhodococcus sp./fermentation/optimization分类
生物科学引用本文复制引用
王利群,王明慧,汪云,卿青,周珏,张月明,胡蓉..一株聚-β-羟基丁酸酯高产菌株的筛选及发酵条件的优化[J].化工进展,2013,(9):2194-2199,6.基金项目
国家自然科学基金（21102011）及常州市生物医药科技专项（CE20115053）项目。 （）
