化工进展Issue(10)：3731-3736,6.DOI:10.16085/j.issn.1000-6613.2015.10.033
表面活化甲壳素固定化Gibberella intermedia生物转化制备异烟酸
Biosynthesis of isonicotinic acid by surface activated chitin immobilized Gibberella intermedia
摘要
Abstract
Chitin and its derivatives have wide sources and biosafety,which showed well application prospect in the fields of material,food,and chemical industry. This study firstly attempted to immobilize nitrilase-producing resting cells ofG. intermedia using surface activated chitin as the material. The immobilization conditions were primarily optimized. 3% of modified chitin,7% of sodium tripolyphosphate,5h of immobilization time and 20g/L of biomass were used as the suitable condition with the highest nitrilase activity displayed. The catalytic performance of immobilized cells was characterized for 4-cyanopyridine biotransformation. The results showed that the optimum temperature,pH,and substrate concentration were 50℃,7.0,and 125mmol/L,respectively. Also, the maximum tolerance toward product concentration was 400mmol/L. These characteristics of immobilized cells demonstrated obvious advantages over corresponding resting cells. Furthermore,the immobilized cells were used for the biotransformation of 4-cyanopyridine into isonicotinic acid. The immobilized cells could be reused for 14 batches,while the resting cells only conducted 3 batches.关键词
甲壳素/异烟酸/4-氰基吡啶/固定化/生物转化Key words
chitin/isonicotinic acid/4-cyanopyridine/immobilization/biotransformation分类
生物科学引用本文复制引用
龚劲松,李恒,杨涛,钱建瑛,陆震鸣,许正宏,史劲松..表面活化甲壳素固定化Gibberella intermedia生物转化制备异烟酸[J].化工进展,2015,(10):3731-3736,6.基金项目
国家自然科学基金（21406088）、江苏省自然科学基金（BK20140133）、江苏省产学研前瞻性联合研究项目（BY2012062）及国家十二五科技支撑计划（2012BAD33B06）项目。 （）
