化工学报Issue(10)：3811-3819,9.DOI:10.11949/j.issn.0438-1157.20150935
核糖核酸开关用于微生物细胞工厂的智能与精细调控
Intelligent and fine regulation of microbial cell factory based on riboswitches
摘要
Abstract
Construction of the microbial cell factory is one of the developmental directions of current green chemical industry. The microbial cell factory is a kind of recombined microorganism and its metabolic and regulatory pathways have been reconstructed by metabolic engineering and synthetic biology to synthetic new compounds or to improve the yield of target production. The microbial metabolic pathway is regulated by two points: environment and genetic information. The cell maintains its homeostasis by global transcription factors, messenger molecules and feedback inhibition when the circumstance is changed. Meanwhile, the cell is affected by its own genetic circulate through transcription, translation and post-translational modification to regulate the expression of target gene. The riboswitches are RNA elements which change their conformation when bind to specific ligands such as ions, sugar derivatives, amino acids, nucleic acid derivatives and coenzymes to regulate the process of transcription, translation and splicing of mRNA. The riboswitches are natural biosensors and bioeffectors which can be designed as the intelligent molecular tools to fine regulate microbial cell factories. Using riboswitches in the microbial cell factory can extend the application in the field of chemical, pharmaceutical, environmental protection and food production.关键词
核糖核酸开关/微生物细胞工厂/精细调控/生物转化/优化/代谢工程/合成生物学Key words
riboswitch/microbial cell factory/fine regulation/biotransformation/optimization/metabolic engineering/synthetic biology分类
化学化工引用本文复制引用
赵雨佳,张根林,周晓宏,李春..核糖核酸开关用于微生物细胞工厂的智能与精细调控[J].化工学报,2015,(10):3811-3819,9.基金项目
国家杰出青年科学基金项目（21425624）；国家自然科学基金面上项目（21376028，21476026）。@@@@supported by the National Science Fund for Distinguished Young Scholars（21425624）and the National Natural Science Foundation of China(21376028,21476026) （21376028,21476026）
