黑龙江农业科学Issue(1)：11-13,14,4.
不同因素对水稻愈伤组织形成的影响
Effect of Different Factors on Rice Callus Formation
摘要
Abstract
Taking Kenjiandao No .10 as the experimental materials ,using 75% alcohol ,0 .1% mercuric chloride and 5% sodium hypochlorite as disinfectants ,the effects of different disinfectants and treatment time on the in-duction of rice mature embryo callus were studied .Meanwhile using the best screening sterilization conditions , the best induced callus matrix were also studied for the selected medium of MS ,B5 ,N6 and the NMB .The re-sults showed that the best disinfection treatment combination was 75% alcohol 1 min + 0 .1% mercuric chlo-ride 10 min + 10% sodium hypochlorite 4 min ;after analysis of variance ,B5 medium was better than other ma-trix on the callus induction of Kenjiangdao No .10 ,callus per dish on B5 medium was higher than other sub-strates ,and the induced callus were large and fresh .关键词
水稻/愈伤组织/影响因素Key words
rice/callus/factors分类
农业科技引用本文复制引用
王玉洋,蒋柳青,董雪梅,孙冬梅..不同因素对水稻愈伤组织形成的影响[J].黑龙江农业科学,2013,(1):11-13,14,4.基金项目
黑龙江省教育厅资助项目(1152G023) （1152G023）
