黑龙江农业科学Issue(8)：14-17,4.
河池报春苣苔的无菌播种、组织培养和快速繁殖
Aseptic seeding,Tissue Culture and Rapid Propagation of Primulina hochiensis
摘要
Abstract
The techniques of tissue culture and rapid propagation of Primulina hochiensis were studied by ex-plants ,comparing with different types and concentrations of plant growth regulators ,types of rooting medium and transplanting medium .The results showed that the better suitable procedure for sterilization was to soak explants in 0 .1% HgCl2 solution for 15 min after pretreatment with 75% alcohol for 1 min ,the seeds were the best explants . 1/2MS+NAA0 .1 mg·L-1 +6-BA0 .15 mg·L-1 could effectively induce fasciculate buds ,MS+6-BA0 .5 mg·L-1 +IBA0 .1 mg·L-1 or MS was suitable for subculture and proliferation multiples was 3~4 times every 30 d;the rooting medium was 1/2MS+IBA 0 .1 mg·L-1 ,and the rooting rate was 100% .The rooting seedlings were effectively transplanted in mixed matrix soil(sawdust∶garden soil∶sand=7∶2∶1) ,the highest survival rate was 90% after a month .关键词
河池报春苣苔/无菌播种/组织培养/快速繁殖Key words
Primulina hochiensis/aseptic seeding/tissue culture/rapid propagation分类
农业科技引用本文复制引用
覃国乐,覃文渊,温放,黄荣明,袁泉..河池报春苣苔的无菌播种、组织培养和快速繁殖[J].黑龙江农业科学,2013,(8):14-17,4.基金项目
广西自然科学基金资助项目（2012 GX-NSFBA053056）；河池学院科研课题资助项目 （）
