哈尔滨商业大学学报（自然科学版）Issue(5)：513-515,526,4.
昙花中槲皮素、异鼠李素和山奈酚量的测定
Simultaneous determination of quercetin, kaempferol and isorhamnetin in Epiphyllum oxypetalum by HPLC
摘要
Abstract
A rapid and sensitive HPLC method was developed to detect the content of querce-tin, kaempferol and isorhamnetin in Epiphyllum oxypetalum .Diamond -C18 column was used as chromatographic column , methanol-0.4%phosphoric acid (55∶45) water solution as mobile phase , and the detection wavelength was set at 360 nm, with a flow rate at 1.0 mL/min.There was a good linear relationship when quercetin was in the range of 5.5~88μg/mL, r=0.999 9(n=6), kaempferol was in the range of 4~64 μg/mL, r=0.999 9(n=6) and isorhamnetin was in the range of 6.5~104 μg/mL, r=0.999 9(n=6).The av-erage recoveries were (99.84 ±0.23)% ( RSD=0.24%), (100.20 ±0.52)% ( RSD=0.38%), 99.99 ±0.12% (RSD=0.11%) respectively.The result showed that the con-tent of quercetin, kaempferol and isorhamnetin in Epiphyllum oxypetalum were 0.05, 0. 026, 0.039 mg/g, respectively.The established method was simple , sensitive and repro-ducible to detect the content of quercetin , kaempferol and isorhamnetin in Epiphyllum oxyp-etalum at the same time , which could be applied in the quality control of Epiphyllum oxyp-etalum and its preparations .关键词
昙花/槲皮素/山奈酚/异鼠李素/高效液相色谱法Key words
Epiphyllum oxypetalum/quercetin/kaempferol/isorhamnetin/HPLC分类
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邹翔,盛洁静,曲中原,张振国,徐阳,季宇彬..昙花中槲皮素、异鼠李素和山奈酚量的测定[J].哈尔滨商业大学学报（自然科学版）,2015,(5):513-515,526,4.基金项目
国家自然科学基金青年基金项目（81102858）；黑龙江省普通本科高等学校青年创新人才培养计划（UNPYSCT－2015070）；黑龙江省博士后资助项目（LBH－Z11102）；哈尔滨商业大学大学生创新创业训练计划国家级项目 （）
