哈尔滨商业大学学报（自然科学版）Issue(5)：536-539,4.
RP －HPLC 法测定北五味子中6种木脂素类成分
Simultaneous determination of content of 6 kinds of lignans in Fructus schisandrae chinensis by RP-HPLC
吴伦 1苏阳 2范海潮 3刘杰 1田振坤1
作者信息
- 1. 黑龙江中医药大学中医药研究院，哈尔滨150040
- 2. 黑龙江中医药大学药学院，哈尔滨150040
- 3. 宜康 杭州 生物技术有限公司，杭州310011
- 折叠
摘要
Abstract
In this paper , RP-HPLC to simultaneously determine 6 kinds of lignans in fruc-tus schisandrae chinensis was adopted .The column was Agilent ZORBAX SB -C18 (5 μm, 4.6 ×150 mm).The mobile phase was consisted of methanol -water solution(gradient elu-tion).The detect wavelength was set at 254 nm, and the column temperature was 25 ℃. Results indicated that deoxyschizandrin at the range of 40.4~600.0μg/mL (r=0.999 8),γ-Schisandrin at the range of 42.0~420.0 μg/mL (r=0.999 9), Schisandrin C at the range of 15.8~118.8μg/mL (r=0.999 5), Schisandrin at the range of 10.0~160.0μg/mL (r =0.999 9), Schisandrol at the range of 15.5 ~388.0 μg/mL ( r =0.999 5) Schisantherin A at the range of 16.3~408.0 μg/mL (r=0.999 9) have good linear rela-tionship between peak area and concentration of reference substance ( n=6 ) .The sample recovery rate of 6 kinds of ingredients was higher than 98%, and the RSD value was less than 3%.This method was simple , accurate , celerity and good reproducibility , and can be used for quantitative analysis of effective ingredients of fructus schisandrae chinensis.关键词
北五味子/木脂素/高效液相色谱法/含量测定Key words
fructus schisandrae chinensis/lignans/RP-HPLC/content determination分类
医药卫生引用本文复制引用
吴伦,苏阳,范海潮,刘杰,田振坤..RP －HPLC 法测定北五味子中6种木脂素类成分[J].哈尔滨商业大学学报（自然科学版）,2015,(5):536-539,4.
