中医药导报Issue(3)：77-79,3.
葛根素对大鼠成骨细胞增殖影响的体外实验研究
Effect of Puerarin on Proliferation of Rat Osteoblast in Vitro
王海珍 1贠云飞 2苗志勃1
作者信息
- 1. 唐山市协和医院，河北 唐山 063000
- 2. 唐山市迁西县人民医院，河北 唐山 064300
- 折叠
摘要
Abstract
Objective: To explore the effect of Puerarin on the proliferation of rat osteoblast in vitro. Methods: Newborn SD rat OBs were separated and cultured in vitro with the calvaria. The OBs of the third generation were cultured. The morphological characteristics of OBs were observed by inverted phase contrast microscope. Immunohistochemistry was used to examine the expression of typeI collagen. Alkaline phosphatase(ALP) and calcifying nodule were stained. The osteoblasts cultured for 1, 3, 5 and 7 day were divided into experimental group at the culture medium doses ranging from 1í10-5 mol/mL to 1í10 -10 mol/mL Puerarin and control group at the common culture medium without any Puerarin. The growth and proliferation ability was analyzed by CCK-8 method and growth curve was drawn. Results: For the OBs growth and proliferation ability by CCK-8 method, three days later, the cells grew fast obviously. The proliferation of cells ranging from 1í10-10 mol/L to 1í10-7 mol/L Puerarin was accelerated significantly after cultured for 5 and 7 days. Conclusion: Puerarin promotes proliferation of OBs and bone formation by the meditation of estrogen receptor. The osteoblasts of OBs treated doses ranging from 1í10-10 mol/L to 1í10-7 mol/L Puerarin was the most accelerated significantly.关键词
葛根素/大鼠/成骨细胞/增殖/体外实验Key words
Puerarin/Rat/Osteoblast/Proliferation/Vitro experiment分类
医药卫生引用本文复制引用
王海珍,贠云飞,苗志勃..葛根素对大鼠成骨细胞增殖影响的体外实验研究[J].中医药导报,2013,(3):77-79,3.
