华侨大学学报（自然科学版）Issue(6)：693-697,5.DOI:10.11830/ISSN.1000-5013.2015.06.0693
巨尾桉胞质 EuCuZnSOD 基因的克隆与原核表达
Cloning and Expression of the Cytosolic Copper/Zinc Superoxide Dismutase Gene in Eucalyptus grandis ×E .ophylla
摘要
Abstract
Copper Zinc superoxide dismutases are avital antioxidant enzymes that catalyze the disproportionation of su-peroxide anion to oxygen (O2 )and hydrogen peroxide (H2 O2 )to guard cells against superoxide toxicity.The cytosolic CuZnSOD gene was cloned from Eucalyptus grandis ×E.ophylla (GenBank Accession Number:JX138573).The cDNA nucleotide sequence analysis revealed that an open reading frame contains 459 bp nucleotide coding for 152 residues (15.2 ku).The full-length gene is amplified to construct expression vetor pET-CuZnSOD .The Escherichia coli is induced by 1 mmol·L-1 IPTG in 28 ℃ for 4 hours and enzyme activity assay result shows that enzyme activity has increased 19.9%than the control in 28 ℃.关键词
巨尾桉/EuCuZnSOD 基因/铜锌超氧化物歧化酶/克隆/原核表达Key words
eucalyptus grandis/copper/Zinc-superoxide dismutases/clone/prokaryotic expression分类
农业科技引用本文复制引用
赵艳玲,周利建..巨尾桉胞质 EuCuZnSOD 基因的克隆与原核表达[J].华侨大学学报（自然科学版）,2015,(6):693-697,5.基金项目
国家自然科学基金资助项目 （）
