海洋科学Issue(2)：1-6,6.
逆转座子序列插入--海湾扇贝过氧化物还原酶V第1内含子分析
A retrotransposon-like insertion-Characterization of Intron 1 in peroxiredoxin V from bay scallop Argopecten irradians
摘要
Abstract
Intron 1 (I1) of peroxiredoxin V (PrxV) from bay scallop was amplified following the genome walking method with primers designed based on the known PrxV cDNA and genomic DNA sequences. Blast, DNAMAN and RepFind programs were used for the analysis of AiPrxV I1. The length of AiPrxV I1is 8 565 bp, which is 68%of the total AiPrxV length and the AT% is significantly higher than GC%. There is an incomplete coding region existing in the 5 900~6 700bp region, which shares high similarity with the reverse transcriptases from amphioxus and sea urchin. In addition, the N terminal sequence of the coding region is similar with the C terminal sequences of several reverse transcriptases involved in retrotransposons. Moreover, 283 repeated sequences were found in AiPrxV I1, which are mainly located in the regions of 3 500~4 000 bp and 6 800~7 200bp. The characteristics in-dicate that the AiPrxV I1 of bay scallop might originate from a non-long terminal repeat (non-LTR), a type of retrotransposable elements.关键词
海湾扇贝(Argopecten irradians)/过氧化物还原酶V/内含子/逆转座子/非长末端重复元件Key words
Bay scallop (Argopecten irradians)/Peroxiredoxin V/Intron/Retrotransposon/non-LTR分类
农业科技引用本文复制引用
李娟,李莉,张国范..逆转座子序列插入--海湾扇贝过氧化物还原酶V第1内含子分析[J].海洋科学,2013,(2):1-6,6.基金项目
国家公益性行业(农业)科研专项(3-35) （农业）
国家自然科学基金项目(30800842) （30800842）
国家高技术研究发展计划项目(863计划,2010AA10A401) （863计划,2010AA10A401）
