海洋科学Issue(2)：76-83,8.
海洋细菌脂肪酸的气相色谱分析
Analysis of Cellular Fatty Acid Composition of Marine Bac-teria by Gas Chromatography
摘要
Abstract
The composition and percentage of fatty acids in Vibrio parahaemolyticus VP-X-3 were analyzed by gas chromatography. With quantity, peak height and percentage of fatty acids showed in the chromatography as the standard, the saponification and methyl esterification conditions were also investigated to give an optimum pre-treatment method. The results indicated that both saponification and methyl esterification influenced fatty acids composition and percentage. The peak quantity and height were increased by lowering saponification temperature. Too high methyl esterification temperature would easily damage fatty acids, especially the structure of long-chain fatty acids and unsaturated fatty acids. The esterification effect would be worse with longer esterification time at higher esterification temperature. The optimum gas chromatography pretreatments were as follows:saponification, 2 mL 2 mol/L NaOH-methanol, water bath at 70 for 10 min;methyl esterification, 2 mL 10%H℃ 2SO4-methanol, water bath at 70 for 15 min. On the chromatogram of GC, the quantity of chromatographic peak was as many as℃29, and the peak height was distinctly higher than those with other different pretreatments.关键词
副溶血性弧菌/脂肪酸/气相色谱/甲酯化/皂化Key words
Vibrio parahaemolyticus/Fatty acid/Gas chromatography/Methyl esterification/Saponification分类
生物科学引用本文复制引用
徐敏,王静,柴子涵,牟海津..海洋细菌脂肪酸的气相色谱分析[J].海洋科学,2013,(2):76-83,8.基金项目
国家科技支撑计划“海洋水产食品加工技术研发与产业化示范(2012BAD28B05)资助 （2012BAD28B05）
