海洋科学Issue(3)：38-44,7.
赤点石斑鱼精子的超低温冷冻及短期保存
Cryopreservation and short-term preservation of red spotted grouper (Epinephelus akaara) sperm
摘要
Abstract
In this paper, the activation characteristics, cryopreservation program optimization, short-term preserva-tion and fertilization of red spotted grouper sperm were studied in detail. The results showed that seawater with specific gravity of 1.018 and temperature of 25℃ has the best activating effect and the sperm motility was (82.50±4.18)%. Frozen samples with 7 cm height above the liquid nitrogen surface reached a post-thaw motility of (65.83±3.76)%, which was significantly higher than other samples (P<0.0001). Using cryopreservation solution B (containing 30 g/L trehalose, 10%DMSO and saline), the post-thaw motility was (67.92±3.96)%, which is signifi-cantly higher than those using the other two cryopreservation solutions. Samples frozen with optimized program (cryopreservation solution B, height of 7 cm) could achieve good fertilization rate (74.55±4.31)%, which was sig-nificantly lower than that of the corresponding fresh sperm (87.42±4.63)%, P<0.05). After two week’s preservation at 4℃, the red spotted grouper sperm motility reduced to 0.关键词
赤点石斑鱼(Epinephelus akaara)/精子/超低温冷冻/低温保存Key words
Sperm of red spotted grouper (Epinephelus akaara)/sperm/cryopreservation/short-termpreservation分类
农业科技引用本文复制引用
郑乐云,黄种持,危林丹,黄长江,董巧香..赤点石斑鱼精子的超低温冷冻及短期保存[J].海洋科学,2013,(3):38-44,7.基金项目
福建省石斑鱼种业创新与产业化工程建设资助项目(闽种石斑鱼002) （闽种石斑鱼002）
