海洋科学Issue(3)：45-49,5.
酶解蓝圆鲹蛋白制备降血压肽的工艺研究
Study on technology of antihypertensive peptides production from Decapterus maruadsi protein with enzymatic hydrolysis
摘要
Abstract
The protein from Decapterus maruadsi was hydrolyzed by six different proteolytic enzymes in their op-tional conditions. The inhibition percent (IP) of Angiotensin I-converting enzyme (ACE) by the hydrolysates was examined with high performance liquid chromatography. Results showed the papain was the best enzyme for pro-ducing ACEI. When the degree of hydrolysis (DH) was 13.7%, the inhibition of ACE by the hydrolysate reached as high as 67.4%. Further on, the influence of hydrolysis time, initial enzyme concentrations ([E]), initial substrate concentrations ([S]), hydrolysis temperatures and pH values was investigated. Results showed that the optimal con-dition for producing ACEI was as follows:[E] at 7000 U/g·Pro, [S] at 25 g/L, hydrolysis temperature at 45℃, pH at 7.0 and hydrolysis time in 240 minutes. Then ultrafiltration technology was used to separate and enrich the active part of hydrolysates. Results showed that the higher active part of the hydrolysates can be enriched in the penetrat-ing fluid of both 10 and 5 KDa. It was also found that the higher active hydrolysates were mainly lower than 5 KDa with an ACE inhibition rate of 88.47%.关键词
酶解/蓝圆 (Decapterus maruadsi)/蛋白/降血压肽鲹Key words
hydrolyze/Decapterus maruadsi/protein/antihypertensive peptides分类
农业科技引用本文复制引用
舒冰,孙建华,伍善广,赵钟兴,廖丹葵..酶解蓝圆鲹蛋白制备降血压肽的工艺研究[J].海洋科学,2013,(3):45-49,5.基金项目
广西科技攻关项目(桂科攻0992025-17 （）
10123008-20) （）
