海洋科学Issue(3)：102-108,7.
杂色鲍血淋巴双向电泳与质谱分析
Two-dimensional electrophoresis and mass spectrometry analysis of Haliotis diversicolor hemolymph
摘要
Abstract
Hemolymph of abalone was used as the material for the two-dimensional electrophoresis research. At first, protein preparation and quantification method based on the acetone precipitation and the RC DC Protein Assay Kit was successfully set up. Then pre-treatment methods with 500 r/min low-speed centrifugation and 35000 r/min ultracentrifugation were used for the separation of hemocyte and blood plasma or reducing the high molecular weight region’s background. Furthermore, non-linear strips (18 cm, pH 3-10) and standardized electrophoresis pro-tocols were established to evaluate different sample volumes with silver stain. Good two-dimensional electrophore-sis maps were finally acquired, and four protein spots in the map were successfully identified by mass spectrometry, which are actin, tropomyosin, collagen and hemocyanin.关键词
杂色鲍(Haliotisdiversicolor)/血淋巴/血浆/双向电泳/蛋白质组Key words
abalone/hemolymph/blood plasma/two-dimensional electrophoresis/proteome分类
农业科技引用本文复制引用
韩焘,姜敬哲,王江勇..杂色鲍血淋巴双向电泳与质谱分析[J].海洋科学,2013,(3):102-108,7.基金项目
现代农业产业技术体系建设专项资金-贝类产业技术体系科学家岗位专项经费项目(CARS-48) （CARS-48）
中央级公益性科研院所基本科研业务费专项资金项目(2010TS16) （2010TS16）
