海洋科学Issue(7)：7-12,6.
乳酸菌及其代谢产物对刺参幼体肠道菌群和非特异性免疫的影响
The influence of lactic acid bacteria and metabolites on intes-tinal microflora and nonspecific immunity of juvenile sea cu-cumber (Apostichopus japonicus)
摘要
Abstract
A 60-day feeding experiment was conducted to evaluate the effects of lactic acid bacteria and its me-tabolites on bacteria of intestine, nonspecific immunity and growth of juvenile sea cucumber with initial body weight of (2.74 ± 0.17) g. Lactic acid bacteria and metabolites were added in the basal diet with 2 mL/m3 (translated by farming water). Compared with control group, the lactic acid bacteria and metabolites added to the feed signifi-cantly reduced the number of intestinal Vibrio and E. coli (P<0.05). The diet containing lactic acid bacteria and metabolites can increase the activities of acid phosphatase, alkaline phosphatase, and lysozyme significantly. There was no significant difference in activities of immune enzyme between the experimental groups. The results showed that the specific growth rate (RSG) of sea cucumber in the experimental groups were significantly higher (P<0.05) than that of the control group. These results indicated that both lactic acid bacteria and metabolites play an impor-tant role on intestinal microflora balance, nonspecific immunity and growth of sea cucumber, but the difference between the experimental groups was not significant. The metabolites may play a major role of probiotics in the process of sea cucumber farming.关键词
刺参(Apostichopus japonicus)/乳酸菌/肠道菌群/非特异性免疫/特定生长率Key words
Apostichopus japonicus/lactic acid bacteria/intestinal microflora/nonspecific immunity/RSG分类
农业科技引用本文复制引用
宫魁,王宝杰,刘梅,蒋克勇,邱楚雯,骆作勇,范瑞用,王雷..乳酸菌及其代谢产物对刺参幼体肠道菌群和非特异性免疫的影响[J].海洋科学,2013,(7):7-12,6.基金项目
中国科学院知识创新工程重要方向项目(KZCX2-EW-Q215)；“十二五”国家科技支撑计划项目(2011BAZ02709) （KZCX2-EW-Q215）
