海洋科学Issue(7):24-32,9.
基于COI和ITS-1基因对魁蚶野生群体和人工繁育子代的遗传分析
Genetic analysis of wild ark shell (Scapharca broughtonii) populations and the artificially propagated progenies based on COI and ITS-1 gene
摘要
Abstract
The genetic variance and population genetic structure of wild S. broughtonii from Rongcheng (RC) and Jimo (JM), as well as Jimo’s artificially propagated progenies (JZ) were analyzed by mitochondrial COI and ribo-somal ITS-1 gene sequences. The feasibility of sowing the Jimo progenies to Rongcheng water for the enhancement of S. broughtonii is discussed. Analyses of molecular variance revealed that the major portion of the genetic diver-sity was found within populations. The Fst value between the RC and JM population based on COI and ITS-1 se-quences was 0.1678 and 0.1193, respectively, which suggests that moderately genetic differentiation existed. The Jimo’s artificially propagated progenies have lower genetic diversity than the wild populations. And the genetic divergence between Rongcheng and Jimo’s progenies populations is larger than the divergence between wild popu-lations of Rongcheng and Jimo. The population genetic structure of S. broughtonii in Rongcheng will be seriously affected, if the Jimo’s artificially propagated progenies are sowed into Rongcheng water. Thus, sowing the Jimo progenies to Rongcheng water for the enhancement of S. broughtonii needs to be carried out very cautiously, and the parental population size should be as large as possible.关键词
魁蚶(Scapharca broughtonii Schrenck)/遗传多样性/COI基因/ITS-1基因Key words
Scapharca broughtonii/genetic diversity/COI gene/ITS-1 gene分类
农业科技引用本文复制引用
陈桢,邱兆星,王鸿霞,张秀梅,刘保忠..基于COI和ITS-1基因对魁蚶野生群体和人工繁育子代的遗传分析[J].海洋科学,2013,(7):24-32,9.基金项目
海洋公益性行业科研专项经费项目(200805069) (200805069)