海洋科学Issue(9)：44-50,7.DOI:10.11759//hykx20150409001
鳗弧菌感染对斑节对虾免疫相关指标的影响
The non-specific immune related indexes response ofPenaeus monodon toVibrio anguillarum challenge
摘要
Abstract
To investigate the effect ofVibrio anguillarum challenge on the non-specific immune related indexes of Penaeus monodon, healthyP. monodon were injected with sterile 0.86% normal saline andV. anguillarum, respec-tively. Total antioxidant capacity (T-AOC), lysozyme (LSZ), and nitrio oxide synthase (iNOS) activities and nitrio oxide (NO) content in hepatopancreas and gills ofP. monodon were measured at different sampling time point. The results showed that: compared with the control group, after challenged withV. anguillarum, T-AOC activities in hepatopancreas and gills both increased to the highest at 6 and 12 h (P<0.05), respectively, and then decrease gradually. LSZ activities in hepatopancreas and gills increased to the peak value at 6 and 12 h (P<0.05), respec-tively, and then decreased gradually to a lower level than the control at 24 h (P<0.05). NO content increased sig-nificantly to the highest at 6 and 3 h, respectively, then decreased to the lowest at 24 h. Although the NO content in gills showed a downward tendency, it was still significantly higher than the control group (P<0.05). iNOS in hepa-topancreas was sensitive toV. anguillarum challenge, and the activity increased to the highest at 3 h, but iNOS activities in gills began to increase significantly at 6 h and reach the highest at 12 h (P<0.05). The results indicated thatV. anguillarum challenge had significant impact on the immune enzyme activities ofP. monodon. T-AOC, LSZ, NO and iNOS were all sensitive toV. anguillarum challenge, and could be regarded as monitor indexes forVibrio diseases.关键词
斑节对虾(Penaeus monodon)/鳗弧菌/总抗氧化能力/溶菌酶/一氧化氮/一氧化氮合酶Key words
Penaeus monodon/Vibrio anguillarum/total antioxidant capacity/lysozyme/nitrio oxide/nitrio oxide synthase分类
农业科技引用本文复制引用
段亚飞,董宏标,王芸,刘青松,李华,张家松..鳗弧菌感染对斑节对虾免疫相关指标的影响[J].海洋科学,2015,(9):44-50,7.基金项目
国家十二五科技支撑计划(2011BAD13B10) （2011BAD13B10）
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014TS15,2012YD02) （中国水产科学研究院南海水产研究所）
广东省海洋渔业科技与产业发展专项项目(A201501B15, A201508B05) （A201501B15, A201508B05）
