海洋科学Issue(10)：36-44,9.DOI:10.11759/hykx20141105002
一株源自海绵的-葡萄糖苷酶抑制活性菌的鉴定、培养条件优化及活性物质的分离
Identification,culture conditions optimization and active compounds isolation for anα-glucosidase inhibiting bacte-rium from sponges
摘要
Abstract
Aiming at isolating naturalα-glucosidase inhibiting (α-GI) compounds from marine microorganisms, this paper identified anα-GI active bacterium (HY95) which was screened by previous study. Its culture conditions were optimized and theα-GI active compounds were isolated from it. Through the morphological observation and the analysis of 16S rDNA sequence, the active strain of HY95 was identified asBrevibacillus borstelensis.By using single factor analysis and orthogonal test, the culture conditions were optimized as inoculum ratio of 2.5% (V/V), shaker speed of 130 rpm, culture temperature of 28℃, and incubation time of 60 h in the modified marine broth medium with 5.00 g/L peptone, 1.50 g/L yeast extract, 9.725 g/L sodium chloride, and the initial pH of 7.5. The active compounds were separated using the column chromatography, thin layer chromatography (TLC), and high performance liquid chromatography (HPLC) with the guidance of bioactive test. The final purified compound was subjected to H1 nuclear magnetic resonance (1H NMR) and was identified as the Cyclo(Tyr-Phe). Theα-GI activity of the pure compound was verified with inhibition rate of 53.72±4.92%. The results of the present study provide a promising direction and source for efficientα-GI compounds.关键词
α-葡萄糖苷酶抑制活性/菌株鉴定/培养条件优化/活性化合物分离Key words
α-glucosidase inhibiting activity/strain identification/optimization of culture conditions/isolation of active com-pounds分类
海洋科学引用本文复制引用
俞思羽,陈毓遒,姜薇,夏翊腾,靳翠丽,周晓见..一株源自海绵的-葡萄糖苷酶抑制活性菌的鉴定、培养条件优化及活性物质的分离[J].海洋科学,2015,(10):36-44,9.基金项目
国家自然科学基金资助项目(41076097,41106113,41271521) （41076097,41106113,41271521）
教育部科学技术研究重点项目资助项目(211065) （211065）
江苏省自然科学基金(BK2012267) （BK2012267）
江苏省教育厅高校自然科学基金资助项目(11KJB170011) （11KJB170011）
