渔业科学进展Issue(5)：45-54,10.DOI:10.11758/yykxjz.20150507
摄食豆肽对星斑川鲽（Platichthys stellatus）幼鱼肝脏蛋白表达的影响
Effects of Soy Peptide on Protein Expression in Liver of Juvenile Starry Flounder (Platichthys stellatus)
摘要
Abstract
To investigate the effects of soy peptides on hepatic metabolism of juvenile starry flounder (Platichthys stellatus), two isonitrogenous (43.98±0.14% dry weight) and iso-energetic (19.89±0.08 kJ/g dry weight) experimental diets were formulated to contain 0% SPH (control, FM) and 24% SPH (SPH) respectively. Two hundred and forty juvenile starry flounders (initial weight of 5.73±0.36 g) were randomly distributed to 2 groups with three replicates per group (40 fish/replicate). Each group was fed one diet for 50 days before collecting liver samples for analysis. The results showed that 448−470 protein spots were detected in the gel area of pH 4−7 and Mr 14.4−97.4 kDa after 2-dimensional gel electrophoresis. Among them, fourteen hepatic proteins showed over 2-fold difference in expression abundance including 3 structural proteins (intermediate filament protein ON3-like, type II cytoskeletal 8-like isoform X2 andβ-actin) and 11 proteins involved in different metabolism. Nine metabolic proteins and 3 structural proteins were up-regulated by at least 2-fold and other 2 metabolic proteins were decreased by 2-fold in fish fed SPH diet compared those fed FM diet. The present results revealed that SPH affected hepatic metabolism by enhancing synthesis of high density lipoprotein and stress protein, promoting glycolysis metabolism for energy production while reducing synthesis of trypsin. The present study elucidated the global metabolic response of fish liver to soy peptides using comparative proteomics technology and provides a theoretical basis to apply the novel plant proteins in aquatic feed.关键词
豆肽/双向电泳/肝脏蛋白/肝脏代谢/表达丰度Key words
Soy peptide/Two-dimensional electrophoresis/Hepatic protein/Hepatic metabolism/Expression abundance分类
农业科技引用本文复制引用
宋志东,王际英,李培玉,乔洪金,张利民,郝甜甜..摄食豆肽对星斑川鲽（Platichthys stellatus）幼鱼肝脏蛋白表达的影响[J].渔业科学进展,2015,(5):45-54,10.基金项目
烟台市科技发展计划项目(2013ZH348)和国家海洋公益项目(201205025)共同资助。 （2013ZH348）
