渔业科学进展Issue(5)：63-70,8.DOI:10.11758/yykxjz.20150509
滁州鲫（Carassius auratus）线粒体全基因组序列分析及系统进化
Sequence and phylogenetic analysis of the complete mitochondrial genome of Chuzhou Crucian Carp (Carassius auratus)
摘要
Abstract
Chuzhou Crucian Carp (Carassius auratus in Chuzhou) is one of the natural gynogenetic crucian carp originated from Anhui Province in China. It belongs to the genus ofCarassius within the family of Cyprinidae. To investigate the species status of this fish, we performed the direct DNA sequencing on the PCR products of the complete mitochondrial DNA (mtDNA). The total length of the mtDNA was 16581 bp, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a non-coding control region. The composition and location of genes in the mitochondrial genome of Chuzhou Crucian Carp were consistent with those of Cyprinidae fishes published in GenBank. The tRNA genes formed typical secondary structures except for tRNA-Ser (AGY). The COⅠ gene had GTG as its initiation codon, and the other 12 protein-coding genes had ATG as the initiation codon. Among the 13 protein-coding genes, 9 had complete stop codons but COⅡ, ND3, ND4 and Cytb had an incomplete T as the stop codon. Sequence analysis showed that there was high similarity between the mtDNA sequences of Chuzhou Crucian Carp and other species in genusCarassius recorded in GenBank (>94%). The nucleotide composition of Chuzhou Crucaian Carp was 31.6% (A), 26.2% (T), 16.1% (G) and 26.2% (C). We conducted the phylogenetic analysis of the mitochondrial genomes of 9Carassius fishes and 1Cyprinus carpio, based on their nucleotide and amino acid sequences of all protein-coding genes. The results indicated that the phylogenetic relationship betweenC. auratus gibelio in Chuzhou and in Fangzheng was the closest whileC. auratus gibelio in Chuzhou andCarassius carassius was the farthest. Our study may provide basic data and contribute to the classification ofCarassius and Cyprinidae fishes.关键词
滁州鲫/线粒体基因组/系统进化分析Key words
Chuzhou Crucian Carp (Carassius auratus)/Mitochondrial genome/Phylogenetic analysis分类
农业科技引用本文复制引用
胡玉婷,胡王,江河,凌俊,段国庆,潘庭双..滁州鲫（Carassius auratus）线粒体全基因组序列分析及系统进化[J].渔业科学进展,2015,(5):63-70,8.基金项目
安徽省财政专项资金项目(14D0508)、安徽省农业科学院成果推广项目(13E0504)和安徽省“115”产业创新团队(皖人才办[2009]2号)共同资助。 （14D0508）
