基础医学与临床Issue(1)：54-59,6.
Gli2基因沉默逆转肝癌细胞系SMMC-7721的上皮间质转化
The reverse effect of Gli2 gene silencing on epithelial-mesenchymal transition in hepatocellular carcinoma cell line SMMC-7721
摘要
Abstract
Objective To explore the effect and mechanism of Gli2 on EMT and invasion in the hepatocellular car-cinoma cell line SMMC-7721.Methods shRNA of Gli2 and Negative control (NC) shRNA were constructed and transfected into SMMC-7721 cells.shRNA-Gli2 group,shRNA-NC group and blank group were set up .Transwell chambers assay , adhesion experiments were used to detect the ability of invasion ,homogeneous and heterogeneous cells intercellular adhesion of each group .Meanwhile, the qRT-PCR, Western blot were used to examine Gli2, E-cadherin ,N-cadherin and vimentin mRNA and protein expression .Results In different hepatocellular carcinoma cell lines and be along with the increasing ability of the invasion in hepatocellular carcinoma cell lines , Gli2 expres-sion was higher .Compared with the shRNA-NC group and blank control group ,the interfered group extensive cells invasion ability was inhibited ( P <0.05 ) , homogeneous cells intercellular adhesion increased and heterogeneous cells intercellular was opposite ( P <0.05 ) , meanwhile , the expression of E-cadherin was declined significantly ( P<0.05 ) ,the expression of N-cadherin , vimentin raised significantly ( P<0.05 ) .Conclusions Silencing Gli2 gene can reverse the EMT of the hepatocellular carcinoma cell line SMMC-7721 and inhibit cell invasion ,its mecha- <br> nism may be related to the up-regulation of E-cadherin and the dow N-regulation of N-cadherin, vimentin.关键词
Gli2/上皮间质转化( EMT)/SMMC-7721/侵袭/黏附Key words
Gli2/epithelial-mesenchymal transition (EMT)/SMMC-7721 cell/invasion/adhesion分类
医药卫生引用本文复制引用
邓大炜,孔宪炳,王平,于庆三,王强..Gli2基因沉默逆转肝癌细胞系SMMC-7721的上皮间质转化[J].基础医学与临床,2015,(1):54-59,6.基金项目
重庆市科技攻关项目 （）
