基础医学与临床Issue(2)：152-156,5.
高浓度尿素诱导人脑微血管内皮细胞系产生炎性因子
High concentrations of urea induce human brain microvascular endothelial cell line to produce inflammatory cytokines
摘要
Abstract
Objective To explore high concentrations of urea-induced human brain microvascular endothelial cell line( HBMECs) to produce inflammatory cytokines and possible mechanism .Methods HBMECs were incubated in high concentrations of urea or mannitol ( as osmotic control ) for 3,6,12 and 24 hours.Expression of TNF-αand iNOS was observed by immunofluorescence .Western blot analysis was employed to assess the protein expressions of TNF-α, iNOS, COX-2, NF-κB/P65 and p-P65.NO concentration was determined by a commercial NO assay kit . Results Immunofluorescence showed high positive immunostaining of TNF-αand iNOS after incubation in high concentration of urea stimulued as compared with control group .The protein expressions of TNF-α, COX-2 and p-P65 were significantly increased at 3 and 6 hours after high urea treatment (P<0.01), and iNOS was continued to increase from 3 to 24 hours ( P<0.01 ) .Moreover , NO content was increased at 3 hours after high urea treatment ( P<0.05 ) .Conclusions High concentration of urea can induce HBMECs to produce inflammatory cytokines .关键词
尿素/人脑微血管内皮细胞/肿瘤坏死因子α/一氧化氮/核因子-κBKey words
urea/human brain microvascular endothelial cells/TNF-α/NO/NF-κB分类
医药卫生引用本文复制引用
王琦,王弘凯,刘丽君,何菲,汪克建,李晋芳,冉建华..高浓度尿素诱导人脑微血管内皮细胞系产生炎性因子[J].基础医学与临床,2015,(2):152-156,5.基金项目
国家自然科学基金（30500171）；重庆市教委项目（KJ120330，KJ120314）；重庆市卫生局项目（2012-1-038）；重庆市渝中区科委项目（20120202）；重庆市科委项目（stc2011 JJA10030）；重庆医科大学大学生科学研究与创新实验项目（201403）；国家（市）级大学生创新创业训练计划项目 （）
