基础医学与临床Issue(2)：178-182,5.
CCK-8降低LPS诱导的小鼠单核细胞RAW264.7 CHOP／caspase-11／caspase-1的表达
CCK-8 decreases the expressions of CHOP/caspase-11/caspase-1 induced by LPS in mouse monocyte RAW 264.7 cells
摘要
Abstract
Objective To investigate the effect of CCK-8 on CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cells.Methods RAW264.7 cells were incubated with LPS and/or CCK-8 and/or CCK-8 1R blocking agent devazepide for indicated times .CHOP and caspase-11 protein expressions were determined by West-ern blot , caspase-1 activity was analyzed by kit , IL-1βexpression level was analyzed by ELISA .Results Both CHOP and caspase-11 expression were upregulated in LPS-activated RAW264.7 cell, which were inhibited by pre-treatment of CCK-8 .Pre-treatment of devazepide inhibited the effects of CCK-8 significantly .The same effects were found in caspase-1 activity and IL-1βexpression .Conclusions CCK-8 exerts an anti-inflammatory effect by inhib-iting CHOP/caspase-11/caspase-1 expressions induced by LPS in RAW264.7 cell.CCK 1R may contribute to the anti-inflammatory effect of CCK-8.关键词
胆囊收缩素/脂多糖/C/EBP同源蛋白/caspase-11/caspase-1Key words
cholecystokinin/lipopolysaccharides/C/EBP homologous protein/caspase-11/caspase-1分类
医药卫生引用本文复制引用
刘威,段林,许光明,于峰,张晓静,宋宁..CCK-8降低LPS诱导的小鼠单核细胞RAW264.7 CHOP／caspase-11／caspase-1的表达[J].基础医学与临床,2015,(2):178-182,5.基金项目
河北省自然科学基金 （）
