基础医学与临床Issue(2)：208-212,5.
shRNA干扰PLCε1基因对人食管癌Eca109细胞增殖和细胞周期的影响
Effect of shRNA interfering PLCε1 gene on proliferation and cell cycle of human esophageal carcinoma Eca 109 cells
周荣秒 1李宾 1牛朝旭 2王娜 1黄茜 1霍向然 1李琰1
作者信息
- 1. 河北医科大学第四医院河北省肿瘤研究所分子生物学研究室，河北石家庄050011
- 2. 石家庄平安医院外科，河北石家庄050021
- 折叠
摘要
Abstract
Objective To explore the impact of silencing PLCε1 gene on proliferation and cell cycle of esophageal carci-noma Eca109 cells.Methods Three plasmid expression vectors (PLCε11, PLCε12 and PLCε13) were constructed to si-lence PLCε1 gene.A negative control plasmid expression vector (HK) was constructed at the same time to serve as a control .The plasmid expression vectors were transfected into esophageal carcinoma Eca 109 cells by cations liposome . The plasmid expression vector with the best interference effect ( PLCε12 ) was chosen .The study included Eca 109 group , HK group and PLCε12 group .Cell viability of Eca 109 cells was evaluated by MTT assay .The cell cycles were detected by FCM .The mRNA expression of P16 and CyclinD1 gene was measured by RT-PCR.Results The cell vi-abilitys of Eca109 cells in PLCε12 group were 80.73%and 75.88%at 48 and 72 h after transfection , which were significantly lower than that of Eca 109 cells in HK group (P<0.001).The percentage of S phase Eca109 cells in PLCε12 group was lower than that of Eca 109 cells in HK group ( P <0.01 ) , the cell cycle of PLCε12 group Eca109 cells was arrested in G0/G1 phase.The P16 gene mRNA expression of PLCε12 group Eca109 cells was higher than that of HK group Eca 109 cells ( P<0.01 ) .Conclusions Silencing PLCε1 gene may up-regulate P16 gene mRNA expression and then arrest the cell cycle at G 0/G1 phase and so inhibit proliferation of Eca 109 cells.关键词
PLCε1基因/食管癌/RNA干扰/增殖Key words
PLCε1 gene/esophageal carcinoma/RNA interference/proliferation分类
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周荣秒,李宾,牛朝旭,王娜,黄茜,霍向然,李琰..shRNA干扰PLCε1基因对人食管癌Eca109细胞增殖和细胞周期的影响[J].基础医学与临床,2015,(2):208-212,5.
