基础医学与临床Issue(9)：1167-1170,4.
ZNF330促进红系分化
ZNF330 promotes erythroid differentiation
摘要
Abstract
Objective To explore the effects of ZNF330 on erythroid differentiation of K562 cells and underlying mechanism .Methods Realtime PCR was performed to detect the expression of ZNF 330 in K562 cells induced by hemin .After CD34 +cells being infected by the recombination lentivirus ZNF 330-RNAi, Realtime PCR was applied to detect the expression of CD235a and γ-globin.The luciferase report assay was performed to examine if ZNF 330 could act as a trans-acting factor in 293T/17 cells.Co-Immunoprecipitation (Co-IP) was applied in 293T/17 cells to detect the interaction between ZNF 330 and ZNF408 which was involved in mRNA degradation .Results The ex-pression of ZNF330 was up-regulated after hemin treatment .The expression of CD235a andγ-globin decreased after inhibition expression of ZNF 330 had no effect on report gene .Co-Ip in two ways confirmed the direct binding be-tween ZNF330 and ZNF408 .Conclusions ZNF330 can promote erythroid differentiation , and a possible mecha-nism is that ZNF330 inhibits the function of ZNF 408 , a factor that is involved in mRNA degradation , through the interaction between the two proteins .关键词
ZNF330/CD34 +细胞/K562 细胞/红系分化/ZNF408Key words
ZNF330/CD34 +cells/K562 cells/erythroid differentiation/ZNF408分类
医药卫生引用本文复制引用
刘晓玲,包韩乌云,赵华路,肖体先,杜鸿琳,王芳..ZNF330促进红系分化[J].基础医学与临床,2015,(9):1167-1170,4.基金项目
国家自然科学基金 （）
