基础医学与临床Issue(9)：1188-1193,6.
局部湿热联合微波消融对乳腺癌荷瘤小鼠CD4＋CD25＋调节性T淋巴细胞比例和功能的影响
The effect of wet hyperthermia combined with microwave ablation on the proportion and function of CD 4 +CD25 +regulatory T cell in animal model of mouse mammary carcinoma
摘要
Abstract
Objective To investigate the effects of wet hyperthermia combined with microwave ablation on the pro-portion and function of CD 4 +CD25 +regulatory T cell in animal model of mouse mammary carcinoma and explore its significance .Methods An experimental model of mouse mammary carcinoma was established and then treated with microwave ablation on day 14.Local wet hyperthermia was also performed .The proportion of CD4 +CD25 +regula-tory T cells (Tregs) in splenocytes was analyzed by FACS.The relative expression of Foxp3, as well as the expres-sion of CTLA-4 and GITR, on Tregs was determined by real-time PCR assay and FACS respectively .Moreover, the secretion of TGF-βand IL-10 from Tregs also were detected by ELISA .In addition , the suppressive capacity of Tregs on CD4 +CD25 -T cell was also examined by MTT assay .Results The proportion of Tregs in wet hyperther-mia combined with microwave ablation treated group decreased significantly compared with that in wet hyperthermia or microwave ablation treated alone group ( P<0.05 ) .Moreover , the expression level of Foxp 3 on Tregs decreased significantly (P<0.05).Furthermore, the expression of CTLA4 and GITR, as well as the secretion of TGF-βand IL-10, on Tregs also obviously decreased (P<0.05).Finally, the suppressive capacity of Tregs on the proliferation of CD4+CD25-T cells were also significantly impaired ( P<0.05) .Conclusions Wet hyperthermia combined with microwave ablation os more effective than single wet hyperthermia or microwave ablation as a mono -therapy alone on the function of Tregs , which may be closely related to the enhanced suppression of tumor growth .关键词
乳腺癌/微波消融/局部湿热/CD4 +CD25 +调节性T细胞Key words
breast cancer/microwave ablation/wet hyperthermia/CD4 +CD25 +regulatory T cells分类
医药卫生引用本文复制引用
周涯,李永菊,周洪练,任社华..局部湿热联合微波消融对乳腺癌荷瘤小鼠CD4＋CD25＋调节性T淋巴细胞比例和功能的影响[J].基础医学与临床,2015,(9):1188-1193,6.基金项目
国家自然科学基金（31370918）；贵州省科技厅联合基金（黔科合J字LKZ［2013］24号）；贵州省卫生厅项目 （）
