基础医学与临床Issue(9)：1194-1198,5.
雌激素通过ERK-FAK信号通路调节MCF-7乳腺癌细胞失巢凋亡
Estrogen regulates anoikis through extracellular signal-regulated kinase (ERK)-focal adhesion kinase (FAK) signaling in MCF-7 breast cancer cells
摘要
Abstract
Objective To investigate the effects of estrogen ( E2) on the resistance to anoikis and a possible role of extracellular signal-regulated kinse ( ERK)-focal adhesion kinse ( FAK) signaling in the effect of estrogen to under-stand its underlying mechanism .Methods Poly-Hema-coated culture of human breast cancer cell line MCF-7 was used to induce anoikis .Cells were treated with E2 and/or pretreated with MEK or FAK inhibitors .Western blot was used to assess the phosphorylation of ERK and FAK , trypan blue staining and cell counting were employed to evaluate cell viability , and Hoechst staining was used to check apoptosis .Results Suspension culture greatly re-duced cell survival (P<0.01), and exposure of MCF-7 cells to E2 (10 nmol/L) led to a significantly increased resistance to anoikis and survival ( P<0.05 ) as compared to DMSO .Meanwhile , E2 induced increased phospho-rylation of both ERK and FAK .Pharmacological inhibition of MEK with U 0126 ( 10 μmol/L ) reduced E2-in-creased cell survival by 57.48%(P<0.01) and E2-decreased anoikis;Treatment with FAK inhibitor (10μmol/L) attenuated E2-enhanced cell survival by 53.59% ( P<0.01 ) and E2-reduced apoptosis .Conclusions E2 con-tributes to the enhanced cell viability and increased resistance to anoikis in MCF-7 breast cancer cells , and ERK-FAK signaling may be involved in the E 2-stimulated survival during suspension culture of MCF-7 cells.关键词
雌激素/细胞外信号调节激酶/局部黏着斑激酶/失巢凋亡/乳腺癌细胞Key words
estrogen/extracellular signal-regulated kinase/focal adhesion kinase/anoikis/breast cancer cells分类
医药卫生引用本文复制引用
何艳,王丹丹,陈华妹,李勇杰,薛启祥,王旭东..雌激素通过ERK-FAK信号通路调节MCF-7乳腺癌细胞失巢凋亡[J].基础医学与临床,2015,(9):1194-1198,5.基金项目
国家自然科学基金 （）
