基础医学与临床Issue(9)：1243-1248,6.
恒河猴骨髓来源未成熟树突状细胞的培养及鉴定
Culture and identification of immature dendritic cells from rhesus monkey myeloid
摘要
Abstract
Objective To develop a method for culturing dendritic cells ( DC ) from rhesus monkey bone marrow CD34 +cells, and identify the cells by morphological observation and cell surface marker detection .Methods To screen rhesus monkey bone marrow cells using a magnetic activated cell sorting (MACS) system then to collect highly purified CD34+hematopoietic stem cells .Recombinant granulocyte macrophage colony stimulating factor (GM-CSF), interleukin-4 ( IL-4) and tumor necrosis factor-α( TNF-α) were applied to isolate CD 34+hematopoietic stem cells to induce bone marrow-derived DCs .Morphological change of cells was observed under an electron microscope , and cell surface molecules were detected using flow cytometry after cells were stained with DC surface marker CD 1a and ma-ture DC surface marker CD83.Results Typical DCs with surface villous and dendritic protrusions were identified 6 days after cytokine treatment .92.35%of the cultured cells were DC and 27.61%of the cultured cells were mature DCs ( mDC) .Conclusions We have established a method for culturing highly purified rhesus monkey bone marrow -derived immature DCs (imDC), which may function as a technine plateform to support research in future .关键词
恒河猴/未成熟树突状细胞/免疫耐受Key words
rhesus monkey/immature dendritic cells/immune tolerance分类
生物科学引用本文复制引用
支良,陈鹏,李朝战,李昌亮,关兴,褚朋,阿永俊..恒河猴骨髓来源未成熟树突状细胞的培养及鉴定[J].基础医学与临床,2015,(9):1243-1248,6.基金项目
国家自然科学基金（30960378，81160069）；内设研究机构云南省卫生厅研究项目 （）
