激光生物学报Issue(5)：436-440,5.DOI:10.3969/j.issn.1007-7146.2015.05.007
未照射条件培养基通过影响活性氧水平引起细胞 DNA 损伤
Conditioned Medium from Unirradiated Cells Could Induce DNA Damage by Influence the Level of Reactive Oxygen Species
摘要
Abstract
Conditioned medium transfer is a commonly used experimental means to carry out research about intercellular signal transmission.In the process of research on radiation induced bystander effects,it was found that conditioned me-dium harvested from unirradiated cells could induce DNA damage in recipient cells.The aim of this study is to investi-gate the source of DNA damage.Through evaluating the levels of reactive oxygen species and DNA damage of cells and the cell viability,it was found that after treated with conditioned medium,the levels of reactive oxygen species of cells were up-regulated,the cell viability was reduced and the DNA damage level was raised.These results suggested that compare to completely control cells,conditioned medium could influence the levels of reactive oxygen species of recipient cells.Therefore,the effect of conditioned medium to cells must be considered seriously in the studies using conditioned medium transfer,especially those involved reactive oxygen species and DNA damage.关键词
条件培养基/转培养基/活性氧/细胞活力/DNA 损伤Key words
Conditioned medium/trans-medium/reactive oxygen species/cell viability/DNA damage分类
生物科学引用本文复制引用
徐帅,丁楠,华君瑞,王兵,王菊芳..未照射条件培养基通过影响活性氧水平引起细胞 DNA 损伤[J].激光生物学报,2015,(5):436-440,5.基金项目
国家重点基础研究发展计划（973计划）项目（2010CB834201）；国家自然科学基金资助项目 （）
