井冈山大学学报（自然科学版）Issue(3)：100-106,7.DOI:10.3969/j.issn.1674-8085.2013.03.024
基于ISSR标记的稻属AA基因组遗传多样性分析
ANALYSIS ON GENETIC DIVERSITY OF AA-GENOME ORYZA SPECIES BY ISSR MARKERS
摘要
Abstract
In order to determine genetic diversity of the AA-genome Oryza species (Poaceae), inter-simple sequence repeat (ISSR) markers from a total of 62 rice accessions collected worldwide were analyzed. These accessions encompassed six wild (O. nivara, O. rufipogon, O. barthii, O. longistaminata, O. glumaepatula, and O. meridionalis) and two cultivated (O. sativa and O. glaberrima) species. 21 selected ISSR primers that produced consistent and repeatable banding patterns revealed significant polymorphisms among the 62 rice accessions with an overall gene diversity (DG) of 0.527, indicating the power of ISSR markers in studying genetic diversity in Oryza germplasm. The consensus tree constructed on the basis of the pairwise Jaccard similarity coefficients of the ISSR banding pattern revealed an evident genetic variation relationships of the AA-genome Oryza species with high bootstrap value supports. It is concluded from this study that the Oryza species from different continents possessed close linkages and current classification of the AA-genome Oryza species suggested by Vaughan (1989) remains valid, particularly in relation to that of the Asian wild rice. The knowledge will be useful for the effective utilization of AA-genome wild Oryza species in rice breeding programs.关键词
遗传多样性/ISSR/稻属/AA基因组Key words
genetic diversity/ISSR/Oryza/AA-genome分类
农业科技引用本文复制引用
段世华,郑卓,罗强,龙伟雄,廖佛才..基于ISSR标记的稻属AA基因组遗传多样性分析[J].井冈山大学学报（自然科学版）,2013,(3):100-106,7.基金项目
ThisworkwassupportedbytheNaturalScienceFoundationofJiangxiProvince(2009GZN0071)andtheScience&Technology(S&T)PlanProjectsofJiangxiProvincialEducationDepartment(GJJ12464) （2009GZN0071）
