江汉大学学报（自然科学版）Issue(5)：460-464,5.DOI:10.16389/j.cnki.cn42-1737/n.2015.05.016
4种植物激素对紫苏叶愈伤组织诱导及黄酮积累的影响
Effect of Four Plant Hormone on Callus Induction and Flavonoid Formation in Perilla Frutescen Leaves
摘要
Abstract
In this study ,with the leaves of Perilla frutescen were used as explant ,effect of different concentrations of the hormone on inducing callus and formating flavonoid in Perilla frutescen leaves were studied by single factor and orthogonal test. It was found that the effect order of four kind of hormones to callus induction was 2,4-D>6-BA>NAA>KT and the order to flavonoid formation of the optimum medium for callus induction was MS+6-BA 2. 0 mg/L+2 ,4-D 0. 5 mg/L+KT 0. 6 mg/L+NAA 0. 8 mg/L by orthogonal design ,under the optimum conditions ,the highest indution rate was 83. 3%after 25 days. Furthemore ,the flavonoid formation of callus achieved at 3. 925%,it is much higher than the natural Perilla frutescen leaves ,the experimental results indicate the feasibility of flavonoid formation with tissue engineering.关键词
紫苏叶/植物激素/黄酮/愈伤组织/正交试验Key words
Perilla frutescen/plant hormone/flavonoids/callus/orthogonal test分类
生物科学引用本文复制引用
郭云贵,程晓敏,王泽琼,于娅,韩晓红..4种植物激素对紫苏叶愈伤组织诱导及黄酮积累的影响[J].江汉大学学报（自然科学版）,2015,(5):460-464,5.基金项目
武汉市市属高校产学研项目 （）
