Abstract
Objective To establish a method for simultaneous determining the content of paeoniflorin,baicalin,berberine hydrochloride,wogonoside,baicalein and wogonin in Kuntai capsule by RP-HPLC wavelength switching method. Meth-ods RP-HPLC wavelength switching method was used to simultaneous determination of 6 components in Kuntai cap-sule.Waters symmetry C18 (5 μm,4.6 mmí250 mm) was used as chromatographic column,and acetonitrile (A)-4%glacial acetic acid (B) was used for moving phase.From 0 to 8 minutes,A (15%-24%);from 8 to 20 minutes,A (24%-31%);from 20 to 35 minutes,A (31%-52%) and from 35 to 40 minutes,A (52%-15%) were used for gradient elution.The volume flow was 1.0 ml/min.From 0 to10 minutes,230 nm was used as ultraviolet detection wavelength.From 10 to19 minutes,280 nm was used as ultraviolet detection wavelength.From 19 to21 minutes,345 nm was used as ultraviolet de-tection wavelength.From 21 to 40 minutes,280 nm was used as ultraviolet detection wavelength.Column temperature was at 30℃and injection volume was 10μl. Results Within the scope of 0.0834-1.668μg (r=0.9993),0.3513-7.026μg (r=0.9995),0.2042-4.084 μg (r=0.9994),0.0735-1.47 μg (r=0.9994),0.0237-0.474 μg (r=0.9993)and 0.0256-0.512 μg (r=0.9993),paeoniflorin,baicalin,berberine hydrochloride,wogonoside,baicalein and wogonin had a good linear relation.The average recovery rate was 99.74%,98.97%,100.71%,100.05%,100.39% and 99.55% respectively.The RSD were 2.51%, 1.76%,1.92%,2.26%,2.57%and 2.54% (n=9) respectively.6 active components was 0.0794,0.3386,0.1952,0.0716,0.0192, 0.0246 mg/ml respectively in Kuntai capsule which batch number was 140403,and all of the RSD less than 3.0%.6 ac-tive components was 0.0812,0.3446,0.1836,0.0642,0.0212,0.0238 mg/ml respectively in Kuntai capsule which batch number was 140803,and all of the RSD less than 3.0%. Conclusion This method had advantages of easy,fast,accuracy, good repeatability and strong specificity.It can be used for quality control of kuntai capsule.关键词
坤泰胶囊/同时测定/波长切换法/高效液相色谱法Key words
Kuntai capsule/Simultaneous determination/Changing wave/High-performance liquid chromatography分类
医药卫生
