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五味子叶枯病病原菌的生物学特性及其拮抗放线菌的初筛∗

王壮 张爱华 雷锋杰 匙坤 张连学

吉林农业大学学报Issue(5):524-529,6.
吉林农业大学学报Issue(5):524-529,6.DOI:10.13327/j.jjlau.2015.2613

五味子叶枯病病原菌的生物学特性及其拮抗放线菌的初筛∗

Biological Characteristics of the Pathogen of Schisandra chinensis Leaf Blight and Screening of Antagonistic Actinomycete Strain against the Pathogen

王壮 1张爱华 2雷锋杰 1匙坤 2张连学1

作者信息

  • 1. 吉林农业大学中药材学院,长春130118
  • 2. 吉林省人参工程技术研究中心,长春130118
  • 折叠

摘要

Abstract

Petri dish culture method was used to study biological characteristics of Schisandra chinensis leaf blight pathogen, and plate confrontation method was used to screen antagonistic actino⁃mycetes. The results showed that mycelia grew faster and spore generated more in medium PDA and PDBA. Temperature affected mycelia growth and spore generation at 25 ℃. At the same tempera⁃ture, dark condition was significantly beneficial for spore generation and light condition was benefi⁃cial for mycelial growth. pH 6~7 was most suitable for the pathogen. Fructose and peptone were the best carbon source and nitrogen source for the pathogen. Deadly temperature for the spore was 54 ℃. Two strains ( A-25-8 and A38 ) of the 28 actinomycetes had potential antagonistic effects against leaf blight pathogen and inhibition rate was 66�26% and 66�10%, respectively.

关键词

五味子/叶枯病/细极链格孢菌/生物学特性/拮抗放线菌

Key words

Schisandra chinensis/leaf blight/Alternaria tenuissmia ( Fr.) Wiltshire/biological characteristic/antagonistic actinomycetes

分类

农业科技

引用本文复制引用

王壮,张爱华,雷锋杰,匙坤,张连学..五味子叶枯病病原菌的生物学特性及其拮抗放线菌的初筛∗[J].吉林农业大学学报,2015,(5):524-529,6.

基金项目

吉林省科技支撑计划重点科技攻关项目(20140520159JH,20130206030YY),国家自然科学基金项目 ()

吉林农业大学学报

OA北大核心CSCDCSTPCD

1000-5684

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