吉林农业大学学报Issue(5)：543-548,6.DOI:10.13327/j.jjlau.2015.2527
大豆花芽差异表达基因片段 GmFBDG01克隆和RNAi表达载体的构建∗
Cloning of GmFBDG01 Gene in Soybean Flowering Bud and Con-struction of RNA Interference Expression Vector
摘要
Abstract
As the first step of reproductive growth of plants, flower bud has an important influence on soybean plant growth and development. Choosing flowering buds in the stage of the seventh com⁃pound leaf in soybean four⁃grain pod mutants of jinong 18 as materials, this study analyzed and com⁃pared results of the trans criptome sequencing. GmFBDG01 core sequence of differential expression gene was cloned by RT-PCR, and soybean RNA interference expression vectors pCPB⁃GmFBDG01⁃RNAi were successfully constructed with intermediate cloning vector pBluescript SK plus. The results will lay foundation for functional verification of related functional genes of soybean four⁃grain pods.关键词
大豆/四粒荚突变体/花芽/差异表达/RNA干扰/表达载体构建Key words
soybean/four-grain pod mutant/flower bud/differential expression/RNA interfer-ence/expression vector construction分类
农业科技引用本文复制引用
潘丽丹,姚丹,王丕武,刘婷婷,郑成忠,赵翠兰..大豆花芽差异表达基因片段 GmFBDG01克隆和RNAi表达载体的构建∗[J].吉林农业大学学报,2015,(5):543-548,6.基金项目
国家自然科学基金项目（30971805），吉林省科技厅重点科技攻关项目 （）
