江苏大学学报（医学版）Issue(5)：369-372,4.DOI:10.13312/j.issn.1671-7783.y150171
腺伴随病毒介导 ADNF-9基因转染对豚鼠卡那霉素致聋的治疗作用
Treatment of adeno-associated virus mediated ADNF-9 gene transfection on guinea pig cochlea deafened by kanamycin
摘要
Abstract
Objective:To observe the effect of rAAV-NT4-ADNF-9 in treating ototoxicity associated with aminoglycoside antibiotics.Methods:The guinea pigs were used,and its auvicle reflex and auditory brain-stem response(ABR)were normal.The ototoxic animal model of guinea pigs were made by the method of hypodemic injection of Kanamycin(400 mg/kg).In animals under general anesthesia,the guinea pigs were cut off the skin of ringt ear sulcus posterior and opened acoustic capsule,then injected the rAAV-NT4-AD-NF-9 10 μL from fossula rotunda.After 14 days,the right acoustic capsule were dislodged.The cochlear were stained with AgNO3 .The cochlear hair cells were observed by contrast phase microscope.Results:Taking the group for rAAV-NT4-ADNF-9 as example,the threshold ABR decreased remarkably after treat-ment(P <0.05).Through the phase contrast microscope the cochlear basilar membrane was complete and the outer hair cells had dot deletion.The thresholds of ABR had no notable differences for the other three groups before and after treatment.Taking comparison between the artificial endolymph control group and empty vector group,through the phase contrast microscope after treatment,hair cells showed a patchy loss in the former group and there was no such phenomenon in the latter group.The average number of hair cells observed in the group of ADNF was significantly higher than that of the artificial endolymph control group and empty vector group(P <0.05).Conclusion:rAAV-NT4-ADNF-9 is able to be transfected into cochle-ar,and to express secretory NT4-ADNF-9 peptide,which preventing hair cells from impairment.关键词
基因治疗/感音神经性耳聋/活性依赖性神经营养因子 9/耳蜗毛细胞/腺伴随病毒Key words
gene theraphy/sensorineural hearing loss/ADNF-9/cochlear hair cell/adeno-associated virus分类
医药卫生引用本文复制引用
景阳,郑国玺,祝康,刘晖,张文..腺伴随病毒介导 ADNF-9基因转染对豚鼠卡那霉素致聋的治疗作用[J].江苏大学学报（医学版）,2015,(5):369-372,4.基金项目
国家自然科学基金资助项目（81271057）；陕西省科技研究发展计划资助项目 （）
