江西农业大学学报2015，Vol.37Issue(5)：798-803,6.DOI:10.13836/j.jjau.2015121
运用RP-HPLC同时测定火龙果中7种有机酸和Vc含量的方法研究
A Study on Simultaneous Determination of 7 kinds of Organic Acids and Vc in Dragon Fruit by RP-HPLC
摘要
Abstract
The main factors that affect the separation of organic acids were studied,and a method for sim-ultaneous and rapid determination of purple red flesh dragon fruit in oxalic acid,tartaric acid,malic acid,acetic acid,lactic acid,citric acid,succinic acid and Vc content with reverse-phase high performance liquid chroma-tography(RP-HPLC) was established.The chromatographic conditions are as follows:the column temperature was 30 ℃,the flow rate was 1 mL/min,the wavelength was 215 nm,the buffer was 0.05 mol/L H3 PO4-Na2HPO4 and a Kromasil column (250 mm× 4.6 mm) was adopted.The results showed that malic acid was the most important organic acid,the next was citric acid.Because this method can complete a determination within10 min.So it has the advantages of fast,high sensitivity and good repeatability,it is a high reference to the de-termination and research of metabolism of organic acid and Vc in fruit.关键词
火龙果/紫红肉/有机酸/Vc/反相高效液相色谱(RP-HPLC)Key words
dragon fruit/purple red flesh/organic acid/Vc/reverse-phase high performance liquid chro-matography(RP-HPLC)分类
农业科技引用本文复制引用
蒋侬辉,凡超,刘伟,向旭..运用RP-HPLC同时测定火龙果中7种有机酸和Vc含量的方法研究[J].江西农业大学学报,2015,37(5):798-803,6.基金项目
国家自然科学基金面上项目(31272135)、广东省科技计划项目(2014B070706018)和广东省实验室体系建设开放运行费(2013B050700006) （31272135）
