江西农业大学学报2015，Vol.37Issue(5)：889-893,5.DOI:10.13836/j.jjau.2015134
赤狐耳成纤维细胞的体外培养及核型分析
Culture of Ear Skin Fibroblasts from the Red Fox (Vulpesvulpes) and Analysis of the Karyotype
摘要
Abstract
This study is to isolate and culture skin fibroblasts from the ear of fox.It propels the research on fox somatic nuclear transfer and transgenic fox.In this study,fox ear skin fibroblasts were obtained by tissue pieced culture.The primary culture was digested by trypsin at a short time for the subculture.The fibroblasts were observed by the electron microscope,which had a good growth status.Then karyotype analysis was conduc-ted on the fibroblasts,which showed a standard karyotype of fox.The results showed that fox ear skin fibroblasts could be cultivated successfully in vitro by means of tissues culture method.The primary culture could be puri-fied and ear fibroblast cell line be formed,which can be used as donor cell for nuclear transfer and propels the research on transgenetic special animals.关键词
赤狐/成纤维细胞/染色体核型Key words
red fox/fibroblast/karyotype分类
农业科技引用本文复制引用
鲍加荣,王世勇,郑军军,杨福合..赤狐耳成纤维细胞的体外培养及核型分析[J].江西农业大学学报,2015,37(5):889-893,5.基金项目
国家重点基础研究发展计划(973计划)项目(2012CB722907) （973计划）
