江西农业大学学报2015，Vol.37Issue(5)：909-913,5.DOI:10.13836/j.jjau.2015138
抗腹泻性贝毒软海绵酸胶体金免疫快速检测技术研究
A Study on Colloidal Gold Immunoassay Technology for Monoclonal Antibody of Okadaic Acid
摘要
Abstract
OA colloidal gold immunoassay technology was studied by adopting self-manufactured diarrhet-ic shellfish poison,okadaic acid (OA) ,monoclonal antibody and BSA conjugate as the colloidal gold labeled substrate.Colloidal gold was prepared with various particle sizes and the colloidal gold with a particle size of 30 nm was determined as the optimal one for labeling anti-OA monoclonal antibody.The conjugate of hapten OA and BSAwas prepared,immunochromatographic strip method for OA rapid detection was established by coating conjugate on nitrocellulose membrane as inspection band,and taking Goat anti Mouse IgG as quality control band based on immune competition law.The method can be used in visual judgment in 3-5 min.The sensitivity is 6.25 ng/mL.According to the process procedure,the immunochromatography rapid assaying card of OA was invented.No instrument and equipment are needed for the card.The OA content threshold value of detecting specimen is 6.25 ng/mL.关键词
腹泻性贝毒/软海绵酸/单克隆抗体/胶体金/免疫监测Key words
diarrhetic shellfish poison/okadaicacid/monoclonal antibody/colloidal gold/immunoassay technology分类
生物科学引用本文复制引用
胡乐琴,马晓康,吴春燕..抗腹泻性贝毒软海绵酸胶体金免疫快速检测技术研究[J].江西农业大学学报,2015,37(5):909-913,5.基金项目
上海市教育委员会科研创新项目(14YZ122)和科促会联盟项目 （14YZ122）
