江西农业学报Issue(7)：50-52,56,4.
农杆菌介导的 Thaumatin基因转化烟草的研究
Transformation of Thaumatin Gene into Tobacco through Agrobacterium Mediation
摘要
Abstract
Tobacco leaves under dark culture for 3 days were used as receptor, and the objective gene Thaumatin was trans-formed by Agrobactorium tumefaciens mediated genetic transformation.The transformation efficiency was affected by several factors , which were optimized.The results showed that infection time of 20 min and Meropenem of 25 mg/L were beneficial to the increase in the transformation efficiency.PPT of 2.0 mg/L was used to screen resistant plant , and the resistant plant was identified by PCR , GUS histochemical staining and Southern blot analysis .These confirmed that the exogenous gene Thaumatin had been integrated into the genome of tobacco.关键词
Thaumatin基因/烟草/根癌农杆菌/遗传转化Key words
Thaumatin gene/Tobacco/Agrobacterium tumefaciens/Genetic transformation分类
生物科学引用本文复制引用
崔波,蒋素华,田云芳,武振江,袁秀云,马杰..农杆菌介导的 Thaumatin基因转化烟草的研究[J].江西农业学报,2013,(7):50-52,56,4.基金项目
河南省郑州市科技攻关资助项目（083STRF40346-2）。 （）
