江西农业学报Issue(7)：88-90,3.
聚丙烯酰胺凝胶固定化酶法制备麦芽四糖的研究
Study on Preparation of Maltotetraose by Using Polyacrylamide Gel Immobilized Enzyme Method
摘要
Abstract
A discontinuous polyacrylamide gel electrophoresis (PAEG) was performed to isolate and purify the maltotetraose -producing amylase from their crude preparations .The bands containing amylase in the gel were detected by mass spectral analysis , and the bands containing high -purity maltotetraose -producing amylase were cut down from the gel .The gel containing maltote-traose-producing amylase was squeezed into small pellets , and then immersed in 10% formaldehyde for the linkage between gel and enzyme as well as the immobilization of the amylase , finally the immobilized maltotetraose -producing amylase was obtained .By the analysis of thin layer chromatography and high performance liquid chromatography , the maltotetraose produced by immobilized enzyme had higher purity than that did by the crude enzyme solution , especially it had a significant decrease in monosaccharide con-tent.In addition, our results also indicated that there was an increase in the specific activity of the immobilized maltotetraose -pro-ducing amylase.关键词
麦芽四糖酶/固定化酶/聚丙烯酰胺凝胶电泳/麦芽四糖Key words
Maltotetraose-producing amylase/Immobilized enzyme/Polyacrylamide gel electrophoresis/Maltotetraose分类
生物科学引用本文复制引用
唐玉,李东霄,梁新红,李元召,孙俊良..聚丙烯酰胺凝胶固定化酶法制备麦芽四糖的研究[J].江西农业学报,2013,(7):88-90,3.基金项目
国家自然科学基金项目（C200101）。 （）
