江西农业学报Issue(8):9-11,3.
油棕基因组 DNA 3种提取方法的比较研究
Comparison of Three Extract Methods for Genomic DNA of Elaeis guineensis
摘要
Abstract
Using CTAB extraction with small sample , PVP method and SDS -CTAB improved method respectively , Genomic DNAs were extracted from the young and mature leaves of Elaeis guineensis Jacq., and were tested by the ultraviolet spectrophotome-ter, agarose gel electrophoresis and PCR reaction .The results showed the extracted total DNA from the young leaves of Elaeis guineensis had the best quality and the highest purity by the CTAB extraction with small sample , but the extracted DNA by other two methods was of poor quality .关键词
油棕/基因组DNA/提取/CTAB小样提取法/PVP法/SDS-CTAB改良法Key words
Elaeis guineensis/Genomic DNA/Extract method/CTAB method/PVP method/SDS-CTAB improved method分类
农业科技引用本文复制引用
周丽霞,肖勇,杨耀东,马子龙..油棕基因组 DNA 3种提取方法的比较研究[J].江西农业学报,2013,(8):9-11,3.基金项目
中国热带农业科学院本级基本科研业务费专项( ITBB120506)。 ()