解放军医学院学报Issue(4)：389-391,404,4.DOI:10.3969/j.issn.2095-5227.2013.04.027
干扰组蛋白去甲基化酶基因表达对Ishikawa细胞增殖凋亡的影响
Interference with JARID1A gene expression on proliferation and apoptosis of Ishikawa cells
摘要
Abstract
Objective To study the interference with siRNA JARID1A gene expression on proliferation and apoptosis of Ishikawa cells in endometrial cancer. Methods Ishikawa cells, transfected with JARID1A siRNA, were divided into sicon group, sicon e2 group, siJARIDIA group, and siJARID1A e2 group. Expressions of progestogen receptor (PR) mRNA and protein in the 4 groups were detected by RT-PCR and Western blot, respectively. Proliferation and apoptosis of Ishikawa cells were assayed by MTT and flow cytometry, respectively. Results Interference with JARID1A could significantly up-regulate the PR expression. E2 could further up-regulate the PR expression and promote the proliferation of Ishikawa cells (P<0.01). The proliferation of Ishikawa cells was inhibited at G1 and G2/M phase 48 h after transfected with siRNA (P<0.05). Conclusion JARID1A siRNA can effectively inhibit the proliferation and apoptosis of Ishikawa cells.关键词
子宫内膜癌/小干扰RNA/组蛋白去甲基化酶Key words
endometrial cancinoma/SiRNA/JARID1A分类
医药卫生引用本文复制引用
郑金丹,刘丽丽..干扰组蛋白去甲基化酶基因表达对Ishikawa细胞增殖凋亡的影响[J].解放军医学院学报,2013,(4):389-391,404,4.基金项目
辽宁省自然科学基金(201102134) （201102134）
锦州市科学技术计划项目(12A1E35) （12A1E35）
Supported by Liaoning natural science fund aids a project financially(201102134) （201102134）
