解放军医学院学报Issue(5)：492-494,521,4.DOI:10.3969/j.issn.2095-5227.2013.05.022
模拟失重致心肌细胞凋亡及其相关机制探讨
Simulated microgravity-induced apoptosis of myocardiocytes and its mechanism
摘要
Abstract
Objective To study the effect of simulated microgravity on apoptosis of in vitro cultured rat myocardiocytes and its mechanism. Methods Primarily cultured new born rat myocardiocytes was used as an experimental material and microgravity effect was simulated with clinostat. Myocardiocyte apoptosis–related molecule c-myc mRNA was detected by RT-PCR after simulated microgravity. Effect of 96 h simulated microgravity on expressions of transcriptional factors c-myc, GATA-4 and MEF2A proteins was detected by western blot. Results The expression level of myocardiocyte apoptosis–related molecule c-myc mRNA and protein was significantly higher whereas the expression level of transcriptional factors GATA-4 and MEF2A was significantly lower in experimental group than in control group after simulated microgravity (P<0.05). Conclusion Clinostat-simulated microgravity up-regulates the apoptosis of cardiomyocytes by down-regulating the expression of transcriptional factors GATA-4 and MEF2A.关键词
模拟失重/大鼠/心肌细胞/细胞凋亡/转录因子Key words
simulated microgravity/rats/cardiomyocytes/apoptosis/transcriptional factor分类
医药卫生引用本文复制引用
王永春,赵疆东,孙喜庆,伍静,张舒,曹新生,王冰,石菲,杨长斌,高原,杜挺媛..模拟失重致心肌细胞凋亡及其相关机制探讨[J].解放军医学院学报,2013,(5):492-494,521,4.基金项目
国家自然科学基金项目(30900279，81171872) Supported by the National Natural Science Foundation of China(30900279,81171872) （30900279，81171872）
