解放军医学院学报Issue(7)：756-759,4.DOI:10.3969/j.issn.2095-5227.2013.07.029
单次大剂量链脲佐菌素诱导的糖尿病模型β细胞凋亡的研究及意义探讨
Single high streptozotocin dose-inducedβ-cell apoptosis in diabetic model and its significance
摘要
Abstract
Objective To elaborate the phasic change ofβ-cell apoptosis in single high streptozotocin (STZ) dose-induced type 1 diabetic model of SD rats. Methods A single high STZ dose (65 mg/kg) was injected into the abdominal cavity of SD rats and their blood glucose and insulin levels were measured. The rats were then sacrificed at the indicated time points and their pancreatic tissue was stained with H&E, immunofluorescence, and TUNEL, respectively. Results The rats showed the high-low-high blood glucose level accompanying the low-high-low insulin level and corresponding pathological changes after they were injected with STZ. Theβ-cell apoptosis (measured with TUNEL staining) increased dramatically 6 hours after STZ injection and sharply reduced 48 hours after STZ injection. TheΒ-cell apoptosis was hardly detected. Conclusion Theβ-cell apoptosis reaches its peak with corresponding changes in blood glucose and insulin levels and in pathological structure of islet 6 hours after a type 1 diabetic model of SD rats is induced with a single high STZ dose.关键词
链脲佐菌素/β细胞凋亡/1型糖尿病模型Key words
streptozotocin/βcell apoptosis/type 1 diabetic model分类
医药卫生引用本文复制引用
..单次大剂量链脲佐菌素诱导的糖尿病模型β细胞凋亡的研究及意义探讨[J].解放军医学院学报,2013,(7):756-759,4.基金项目
国家高技术研究发展计划(863计划)(2011AA020113) Supported by the“863 Projects”of Ministry of Science and Technology of PR China (2011AA020113) （863计划）
