Abstract
Objective To observe the impacts of different treatments of specimen on biochemical tests of some items .Methods The results that specimens were tested right away after collections were put into the matched group .(1) the indices of ALT ,AST ,ALP ,GGT and LDH were determined separately 3 hours ,7 hours and 24 hours after the blood were collected .(2) The indices of ALT ,LDH and GLU were determined separately when the blood specimens and the serum specimens were stored at 4 ℃ for 24 hours .(3) The index of ALP was determined when the blood specimens and the serum specimens were stored in the condition of sealed and unsealed at 4 ℃ for 24 hours . Results ALT of control sample and samples laid side for 3 ,7 and 24 hours were 16 .00 ± 6 .48 ,16 .01 ± 6 .72 ,16 .37 ± 6 .92 ,16 .57 ± 7 .09(P>0 .05) .AST of control sample and samples layed side for 3 ,7 and 24 hours were 18 .3 ± 4 .72 ,18 .2 ± 4 .68 ,19 .1 ± 4 .33 ,17 .8 ± 4 .75(P>0 .05) .ALP of samples layed side for 3 ,7 and 24 hours were 78 .1 ± 16 .28 ,77 .1 ± 15 .82 ,78 .2 ± 16 .55 ,77 .4 ± 16 .15(P>0 .05) .GGT of control sample and samples layed side for 3 , 7 and 24 hours were 11 .4 ± 5 .62 ,11 .3 ± 5 .65 ,12 .0 ± 5 .33 ,12 .0 ± 5 .69 (P>0 .05) .LDH of control sample and samples layed side for 3 ,7 and 24 hours were 183 .8 ± 21 .96 ,183 .3 ± 22 .74(P>0 .05) ,188 .4 ± 25 .15(P<0 .01) , 196 .2 ± 26 .41(P<0 .01) .ALT of control sample ,blood specimens and the separated serum specimens were 11 .29 ± 3 .19 ,12 .14 ± 3 .07 ,11 .41 ± 2 .97(P>0 .05) .LDH of control sample ,blood specimens and the separated serum specimens were 194 .83 ± 27 .09 ,217 .41 ± 28 .35(P<0 .01) ,202 .75 ± 27 .22(P>0 .05) .GLU of control sample , blood specimens and the separated serum specimens were 4 .88 ± 0 .68 ,3 .30 ± 0 .81(P< 0 .01) ,5 .08 ± 0 .80( P>0 .05) .For Sealed storage ,ALT of control sample ,blood specimens and the separated serum specimens were 74 .33 ± 11 .79 ,74 .60 ± 12 .05 ,74 .66 ± 11 .61(P>0 .05) .For unsealed storage ,ALT of control sample ,blood specimens and the separated serum specimens were 70 .80 ± 7 .85 ,85 .86 ± 6 .65(P<0 .01) ,92 .60 ± 5 .67 .Conclusion the time of laying aside ,the blood being stored and the un-covered condition have the impacts on the results .关键词
生化检验/酶/葡萄糖/标本处理/质量控制Key words
biochemical test/enzyme/glucose/Specimen handling/quality control
